Immunomagnetic separation of Escherichia coli O26, O111 and O157 from vegetables

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Immunomagnetic separation of Escherichia coli O26, O111 and O157 from vegetables.

AIMS Raw fruits and vegetables have been increasingly associated with human infections caused by Shiga toxin-producing Escherichia coli. This study evaluates the isolation and detection of E. coli O26, O111 and O157 from vegetable samples using immunomagnetic particles. METHODS AND RESULTS Standard cultivation and immunomagnetic separation (IMS) procedures were compared. It was found that imm...

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Adherence of enterohemorrhagic Escherichia coli O157, O26, and O111 strains to bovine intestinal explants ex vivo.

We used bovine intestinal organ culture to study infection by enterohemorrhagic Escherichia coli serogroups O157, O26, and O111. We show colonization and attaching and effacing lesion formation on explants derived from the ileum, colon, and rectum. Intimin and Tir were detected at the sites of adherent bacteria; Tir was essential for colonization.

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Long-term survival of shiga toxin-producing Escherichia coli O26, O111, and O157 in bovine feces.

Cattle are an important reservoir of Shiga toxin-producing Escherichia coli (STEC) O26, O111, and O157. The fate of these pathogens in bovine feces at 5, 15, and 25 degrees C was examined. The feces of a cow naturally infected with STEC O26:H11 and two STEC-free cows were studied. STEC O26, O111, and O157 were inoculated into bovine feces at 10(1), 10(3), and 10(5) CFU/g. All three pathogens su...

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Shiga toxin-producing Escherichia coli O157, O26 and O111 in cattle faeces and hides in Italy

INTRODUCTION Ruminants are regarded as the natural reservoir for Shiga toxin-producing Escherichia coli (STEC), especially of serogroup O157. MATERIALS AND METHODS During 2011 and 2012, 320 samples (160 faecal samples from the rectum and 160 hide samples from the brisket area) were collected from 160 cattle at slaughter in Northern Italy during warm months (May to October). Cattle were reared...

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To identify the reservoirs of shiga toxin-producing Escherichia coli O157, sensitive detection andisolation methods are necessary. The sensitivity of traditional culture methods can be improved significantlyby the inclusion of an immunoconcentration step, resulting in less false-negative results. In this study,enrichment procedure and immunomagnetic separation (IMS) were compared for use in con...

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ژورنال

عنوان ژورنال: Letters in Applied Microbiology

سال: 2001

ISSN: 0266-8254,1365-2673

DOI: 10.1046/j.1472-765x.2001.00941.x